TABLE 1.
Adsorption and elution of Pan-1 seeded in different types of water followed by passage through a 1MDS filtera
Type of water | % Adsorbed (range) | % Eluted (range) | % Recovery (range) |
---|---|---|---|
Deionized | >99 | 95 (85–104) | 94 (84–103) |
Finished | >99 | 74 (74–75) | 73 (73–74) |
Ground | >99 | 68 (62–73) | 67 (62–72) |
Surface | 86 (85–87) | 74 (66–82) | 64 (57–71) |
Pan-1 was seeded in different types of water at a concentration of 220 PFU/ml. Adsorption of Pan-1 to a 1MDS filter was determined by comparison of Pan-1 titers in the water, as determined by PA, before and after passage through the filter. The recovery rate was determined by dividing the Pan-1 titer in the eluate by the titer seeded. The elution rate was calculated based on the Pan-1 titer in the eluate relative to the estimated amount of Pan-1 adsorbed to the filter. In the case of deionized, finished, and ground water samples, the adsorption rates were estimated to be 99%. Viruses were eluted with 3% BE (pH 9.0).