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. 2022 Jun 14;27(12):3823. doi: 10.3390/molecules27123823

Figure 2.

Figure 2

The effect of liquiritigenin on CP-induced apoptosis of renal tubule epithelial cells. (A) TdT-mediated dUTP nickend labeling (TUNEL) assays were performed to assess renal cell death. Nuclei were revealed using 4′,6-diamidino-2-phenylindole staining. (B) Representative flow charts showed that cell apoptosis was determined by flow cytometric analysis in renal tubule epithelial cells with different treatments. Cells stained with fluorescein isothiocyanate (FITC)-conjugated AnnexinV and propidiumiodide (PI). (C) Quantitative assessment of the TUNEL+ cells (numbers per high-power field) in mice kidneys. * p < 0.05 vs. vehicle-control group, # p < 0.05 vs. CP treatment group (n = 6 mice/group). (D) Quantification of the percentage of apoptotic cells. * p < 0.05 vs. vehicle-control group, # p < 0.05 vs. CP treatment group. Data were obtained from three independent experiments. (E) 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays were performed to assess cell viability in renal tubule epithelial cells with different treatments. * p < 0.05 vs. vehicle-control group, # p < 0.05 vs. CP treatment group. Data were from five independent biological replicates.