Table 1.
20-plex panel of ABNs for pneumonia
| Name | Sequence (reporter, peptide sequence) | Sequence source |
|---|---|---|
| BV01 | e(+2G)(+6V)ndneeGFFsAr -(ANP)- GGAIEFDSGC -(PEG8-40kDa) | Published GZMB substrate (36) |
| BV02 | eG(+6V)ndneeGF(+1F)s(+1A)r -(ANP)- GGHPGGPQC -(PEG8-40kDa) | Commercially available CATK substrate |
| BV03 | e(+3G)(+1V)ndneeGFFs(+4A)r -(ANP)- GGGVFRMLSVGC -(PEG8-40kDa) | Derived GZMA substrate (37) |
| BV04 | e (+2G)Vndnee(+2G)FFs(+4A)r -(ANP)- GGGLFRSLSSGC -(PEG8-40kDa) | Screened GZMA substrate (37) |
| BV05 | eGVndnee(+3G)(+1F)Fs(+4A)r-(ANP)- GGGLLYGKGGC -(PEG8-40kDa) | Published CAPN2 substrate (38) |
| BV06 | e(+2G)(+6V)ndnee(+3G)(+1F)(+1F)s(+1A)r -(ANP)- GGy-Tic-TNGC -(PEG8-40kDa) | Derived LGMN substrate (39) |
| BV07 | eG(+6V)ndnee(+3G)(+1F)Fs(+4A)r -(ANP )- GGfPRSGGGC -(PEG8-40kDa) | (26) |
| BV08 | e(+3G)(+1V)ndneeG(+10F)FsAr -(ANP)- GGGSGRSANAKGC -(PEG8-40kDa) | (26) |
| BV09 | e(+2G)Vndnee(+2G)F(+10F)sAr -(ANP)- GGGIQQRSLGGGC -(PEG8-40kDa) | (21) |
| BV10 | eGVndneeGF(+10F)s(+4A)r -(ANP)- GGIPSIQSRGLGC -(PEG8-40kDa) | Influenza hemagglutinin mimic peptide |
| BV11 | e(+2G)(+6V)ndneeG(+10F)(+1F)s(+1A)r -(ANP)- GGNLARALKQTIGC -(PEG8-40kDa) | Screened MMP substrate (40) |
| BV12 | eG(+6V)ndneeG(+10F)Fs(+4A)r -(ANP)- GGHMVQHLIQWHGC -(PEG8-40kDa) | Screened MMP substrate (40) |
| BV13 | e(+3G)(+1V)ndnee(+2G)(+10F)Fs(+4A)r -(ANP)- GGPRAAA-Homophe-TSPGC -(PEG8-40kDa) | Screened ADAM9 substrate (41) |
| BV14 | e(+2G)Vndnee(+3G)(+10F)(+1F)s(+4A)r -(ANP)- GGTGPPGYTGC -(PEG8-40kDa) | Screened ADAMTS substrate (42) |
| BV15 | eGVndneeG(+10F)(+10F)sAr-( ANP)- GGTGLPVYQGC -(PEG8-40kDa) | Screened ADAMTS substrate (42) |
| BV16 | e(+2G)(+6V)ndnee(+3G)(+10F)(+1F)s(+4A)r-( ANP)- GG-Nle(O-Bzl)-Met(O)2-Oic-Abu-C -(PEG8-40kDa) | Published NE substrate (43) |
| BV17 | eG(+6V)ndneeG(+10F)(+10F)sAr- (ANP)- GGAAFAGC -(PEG8-40kDa) | Published NE substrate (23) |
| BV18 | e(+3G)(+1V)ndnee(+2G)(+10F)(+10F)sAr-( ANP)- GGGGGPGC -(PEG8-40kDa) | (21) |
| BV19 | e(+2G)VndneeG(+10F)(+10F)s(+4A)r- (ANP)- GGPLGMRGGC -(PEG8-40kDa) | (26) |
| BV20 | eGVndnee(+2G)(+10F)(+10F)s(+4A)r-ANP - GGP-(Cha)-G-Cys(Me)-HAGC -(PEG8-40kDa) | (26) |
Each nanoparticle consists of a mass-encoded GluFib reporter (in italics, d-amino acids represented by lowercase letters) attached to a photolabile linker (3-amino-3-(2-nitro-phenyl)propionic acid, ANP). The adjacent peptide substrate (in bold) is conjugated to an inert 40-kDa 8-arm PEG scaffold. All substrate sequences were derived from published studies.