Fig. 5.

Characteristics of Desulfobacteraceae bacteria associated with Globobulimina. (A) Neighbor-net network for Desulfobacteraceae associated with the genus Globobulimina from Sweden and Peru (Globobulimina spp. and G. pacifica, respectively) and additional Deltaproteobacteria. The network is based on a concatenated alignment of 33 single copy orthologous protein sequences found in all isolates shown. Red fonts highlight isolates found in association with members of the genus Globobulimina. The monophyly of the MAG Gpa_30 and MAG Glo_11 suggests a common ancestry. Note that the closest relatives to that pair are two uncultivated MAGs (Deltaproteobacteria bacterium B6_G6 and Desulfobacteraceae bacterium 4572_89) sampled from sediments in deep-sea hydrothermal vent sites at the Gulf of California. (B) Presence of predicted proteins encoding metabolic pathways in representative members of Desulfobacteraceae. Three pathways are shown: Wood-Ljungdahl pathway, (dissimilatory) sulfate reduction and nitrate respiration. Asterisks highlight genomes in draft state. Circles indicate for the presence of homologs identified by the KEGG KAAS server. The thin bars link enzymes that participate in the same reaction step either by being an alternative enzyme or being part of one protein complex. The gray circles indicate for cases that were discarded following a manual inspection.