FIG. 4.

Differential protein spectral mapping of collagen-binding proteins from Lactobacillus biosurfactants by using normal-phase (NP-1) and collagen type VI immobilized preactivated surface (PS-1) ProteinChip arrays. Biosurfactant samples (2 μl) prepared from Lactobacillus strain RC-14 and Shirota were spotted onto the surfaces of either PS-1 arrays containing immobilized collagen type VI (Cn-VI/PS-1) or normal-phase (NP-1) ProteinChip arrays. SELDI-TOF analysis was carried out by using a laser intensity setting of 50. The spectra show relative peak intensities versus mass-to-charge ratios (M/z) of proteins for each of the biosurfactants indicated. The short vertical lines indicate protein peaks. The difference maps show the ratios of the common peak intensities (Cn-VI/PS-1:NP-1) for each biosurfactant. Collagen-binding proteins with peak intensity ratios of >2 (above the dotted line) are indicated by asterisks and numbered. The star identifies the 26-kDa collagen-binding protein.