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. 2022 Jun 10;15:904935. doi: 10.3389/fnmol.2022.904935

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Copyright © 2022 Deng, Wang, Hu, Lin, Li, Liu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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brsk2b mutant zebrafish displayed ASD-like behaviors. (A,D) The experimental paradigm of social preference test at 1.5 and 4 mpf. (B,C) The brsk2b^–/– zebrafish displayed a significantly reduced preference for conspecifics compared to brsk2b^+/+ zebrafish. And the change of social preference index (SPI) for brsk2b^–/– zebrafish was significantly smaller than brsk2b^+/+ fish (brsk2b^+/+: brsk2b^–/– = 17: 17, p = 0.029). Data for each genotype are presented as mean ± SEM and compared with Student’s t-tests. SPI changes are compared by a paired t-test. (E,F) The SPI of brsk2b^–/– zebrafish at 4 mpf was significantly reduced compared to brsk2b^+/+ fish both by distance (E) and time (F) ratio (brsk2b^+/+: brsk2b^–/– = 10: 14, p = 0.014 and 0.012, respectively). (G) The experimental paradigm of the shoaling test at 2.5 mpf. (H) The average inter-individual distance of brsk2b^–/– zebrafish was smaller than that of brsk2b^+/+ fish (brsk2b^+/+: brsk2b^–/– = 9: 10, p = 0.036). (I) The stereotyped swimming patterns in zebrafish are shown as “circling” and “walling.” (J) The brsk2b^–/– zebrafish exhibited a trend of higher frequency of stereotyped behaviors though with no significant difference when compared to brsk2b^+/+ fish (brsk2b^+/+: brsk2b^–/– = 9: 13, p = 0.206 and 0.980, respectively). Data for each genotype are presented as mean ± SEM and compared with Student’s t-tests. *p < 0.05, ****p < 0.0001.