Fig. 1.

snRNA-seq analysis and markers identification of the fly adult kidney. (A) A single UMAP of the “fly kidney” contains 11 distinct cell clusters that were annotated on the UMAP. Note, however, that nephrocytes and tubules are not physically associated in vivo. (B) Expression levels and percentage of cells expressing the marker genes in each cluster are shown as a dot plot. (C and D) GFP expression under the control of Gal4 lines specific for each of the principal cell (PC) and stellate cell (SC) clusters. Note that the Gal4 expression patterns of SPR, Wnt4, Esyt2, Debcl, Octalpha2R, CG30377, and ih have not been previously reported. (Scale bars, 500 μm.) (E) Malpighian tubule cell types are identified based on differentially expressed marker genes.