Fig. 3.
Different requirements of receptors for taste avoidance of different metal ions. (A–D) Mutants of four receptors showed different phenotypes for CuSO4 (A and C) and ZnSO4 (B and D) in feeding (A and B) and egg-laying (C and D) behaviors. (E) Feeding avoidance of other metals (MnSO4, FeSO4, Fe2(SO4)3, CoSO4, NiSO4, AgNO3, CdSO4) showed various dependence on Ir76b and Gr66a. n = 10 to 15 in feeding assay and n = 30 to 32 in egg-laying assay. Asterisks indicate significant differences from control (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) using Mann–Whitney U test. Error bars are SEM. ns, not significant.