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. 2022 Jun 14;119(25):e2204238119. doi: 10.1073/pnas.2204238119

Fig. 4.

Fig. 4.

Physiological responses of taste sensilla in the labellum to metal ions. (A) Representative recordings of responses to 10 mM zinc ions in S-b sensilla of control flies and Ir25a and Ir76b mutants. (B) Action potentials induced by different metal ions at 10 mM in S-a and S-b sensilla. n = 4 to 12. (C) Responses to 10 mM zinc ions of S-b sensilla of control flies and Ir25a and Ir76b mutants. n = 8 to 10. ****P < 0.0001, Mann–Whitney U test. (D and E) Effects of different concentrations of copper or zinc on sugar responses in L sensilla. Mixtures of 50 mM sucrose with increasing concentrations of metal ions were applied to the same L sensilla at 2-min intervals. R denotes “recovery” (i.e., the response to 50 mM sucrose after the sensilla were stimulated with sucrose-metal mixtures). (D) Responses to sucrose and sucrose-copper mixtures from L sensilla. n = 10. **P < 0.01, Wilcoxon test. (E) Responses to sucrose and sucrose-zinc mixtures from L sensilla. n = 6. *P < 0.05, Wilcoxon test. (F) PERs to 50 mM sucrose alone or mixtures containing different concentrations of copper or zinc ions. n = 5 to 8. *P < 0.05, **P < 0.01, Mann–Whitney U test. (G–I) Effects of different concentrations of copper on responses to 1 mM caffeine in S sensilla. (G) Representative recordings of responses to 1 mM caffeine alone or caffeine-copper mixtures in S-b sensilla. (H) Responses to caffeine and caffeine-copper mixtures in S-a sensilla. n = 5 to 12. **P < 0.01, ****P < 0.001, Wilcoxon test. (I) Responses to caffeine and caffeine-copper mixtures in S-b sensilla. n = 5 to 12. *P < 0.05, **P < 0.01, ****P < 0.001, Wilcoxon test. Error bars are SEM.