Fig. 4.

Dystrophin missense mutations alter YAP mechanoactivation. (A) RhoA activation as a ratio of RhoA-GTP over total RhoA, measured by a luminescent enzyme-linked immunosorbent assay–type assay and Western blot, respectively. (B) Nuclear-to-cytoplasmic YAP ratio quantification (n = 3). (C) Representative images of myoblasts immunostained for YAP. Cyan arrow heads show YAP-filled nuclei and magenta arrowheads show nuclei with low YAP staining. (Scale bar, 30 μm.) Large triangles denote the mean of independent experiments, and smaller dots indicate individual measurements. Each experiment and corresponding individual measurements are colored the same. Data are presented relative to corresponding NTg value. Data analyzed via one-way ANOVA; ns, not significant (All error bars, SEM).