Fig 1. Specificity of the developed real-time PCR system for detecting ALV J and K.

A. Primer positioning around the ev-D/EAV-HP (GenBank accession numbers DQ500016 and AC270426) similarity breakpoint in the ALV J gene that codes for the gp85 coat protein. A diagram of the full ALV genome is on top, with the region surrounding primer placement shown in detail below. B. A representative set of detection curves from the Cy5.5 and ROX channels (the KPL and JJPLN probes, respectively, in Table 1). Each curve is a sample from a separate bird. Eight ALV J positive, ALV K positive, and negative samples each were measured on the same 96-well plate and fluorescence values recorded simultaneously. Data for this graph are included as the S1 File.