TABLE 2.
Effect of 5- and 20-h supernatants of transformants in Vero cell cytotoxicity assay
| Incubation time (h)a | Vero cell survival (mean % live cells ± SEM)b with:
|
||
|---|---|---|---|
| Transformed E. coli JM109c | Transformed Salmonella serovar Enteritidisc | Vesiclesd | |
| 5 | 47.3 ± 2.8 | 46.0 ± 3.0 | 64.9 ± 3.2 |
| 20 | 36.4 ± 4.1 | 34.6 ± 3.9 | 68.9 ± 2.6 |
a
Growth time in LB broth following transformation.
b
Percent live cells was calculated as explained in Materials and Methods.
c
Controls for the transformants were Vero cells treated with the appropriate bacteria (supernatant) and incubated for the same time under the same conditions.
d
The control for vesicles was Vero cells treated with LB broth.