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. 2022 Jun 24;17(6):e0269920. doi: 10.1371/journal.pone.0269920

Fig 5. mRNA expression of HSD11B2 in HRMC and HRGEC.

Fig 5

mRNA expression of the enzyme HSD11B2 was assessed by Real-time PCR. Amplification cycle number was 40. Ct values are shown. Gene detection was defined to be positive at ct values <35. HRMC: no detection of HSD11B2 (Undet. = undetected), ct >40. HRGEC: no detection of HSD11B2, ct > 35. HEK293: HSD11B2 detected, ct values ~ 29. JEG-3: HSD11B2 detected, ct values ~ 29. HEK293 and JEG-3 were used as positive controls. *p<0.05, **p<0.01, ***p<0.0001, ns = not significant.