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. 2022 Jun 24;17(6):e0269920. doi: 10.1371/journal.pone.0269920

Fig 7. mRNA expression of TGFB1, FN1 and COL1A1 in HRMC upon aldosterone and cortisol stimulation with and without MR and GR inhibition in high glucose conditions.

Fig 7

A) mRNA expression of TGFB1 in HRMC. Baseline was EtOH. Fold difference is shown with cyclophilin A as endogenous control. Aldosterone (*p = 0.015) and cortisol (**p = 0.004) significantly upregulated TGFB1 mRNA expression. The upregulation of TGFB1 by aldosterone and cortisol could not be changed with spironolactone (Aldo vs. Aldo + spironolactone: ns; cortisol vs. cortisol + spironolactone: ns). The combination Aldo + RU486 (**p = 0.002) and cortisol + RU486 (***p<0.0001) stimulated TGFB1 expression more than Aldo and cortisol alone. B) mRNA expression of FN1 in HRMC. Baseline was EtOH. Fold difference is shown with cyclophilin A as endogenous control. Aldosterone (***p<0.0001) and cortisol (***p<0.0001) significantly upregulated FN1 mRNA expression up to 4-fold. The upregulation of FN1 by aldosterone and cortisol could significantly be reduced by spironolactone (Aldo vs. Aldo + spironolactone: **p = 0.002; cortisol vs. cortisol + spironolactone: ***p<0.0001). RU486 was also able to reduce the upregulation of FN1 by aldosterone and cortisol. Aldo vs. Aldo + RU486 (**p = 0.002) and cortisol vs. cortisol + RU486 (**p = 0.004). C) mRNA expression of COL1A1 in HRMC. Baseline was EtOH. Fold difference is shown with cyclophilin A as endogenous control. Aldosterone (**p = 0.006) and cortisol (***p<0.0001) significantly upregulated COL1A1 mRNA expression. The upregulation of COL1A1 by aldosterone and cortisol could be reduced by spironolactone, although only significant in combination with cortisol (Aldo vs. Aldo + spironolactone: ns; cortisol vs. cortisol + spironolactone: *p = 0.023). RU486 was not able to reduce the upregulation of COL1A1 by aldosterone and cortisol. Aldo vs. Aldo + RU486 (ns) and cortisol vs. cortisol + RU486 (ns). ns = not significant. Mean +/- SD, One-way ANOVA, Dunnett’s multiple comparisons test, n = 3. *p<0.05, **p<0.01, ***p<0.0001, ns = not significant.