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(a) Viability of primary hepatocytes and HUVECs assessed using calcein AM (green) and EthD-1 (red) staining on day 7 of culture; (b) quantified metabolic activities of primary hepatocytes and HUVECs at different periods of culture; (c) F-actin and nuclei staining of primary hepatocytes and HUVECs at day 7 of culture; (d) immunostaining of primary hepatocytes for CYP3A and HUVECs for CD31 and VE-cadherin at day 7 of culture.
