Dose-dependent nisin induction of TTFC expression in L. plantarum NCIMB8826 Int-1(pMEC46). Western blotting was performed on 2 μg of soluble proteins (lanes 2 to 9) from total cell extracts. Lane 1, purified recombinant TTFC; lane 2, L. plantarum NCIMB8826 Int-1 (negative control); lanes 3 to 9, NCIMB8826 Int-1(pMEC46) uninduced and induced with 0.5, 1, 1.5, 2, 5, or 20 ng of nisin/ml, respectively. Sizes as deduced from molecular mass markers are shown at the left. Relative band intensities in lanes 3 to 9 are shown at the top.