TABLE 1.
Growth characteristics of the methanotrophic-heterotrophic community and its methanotrophic strain
| Initial headspace concn (%)
|
Growth
rate/daya
|
||||
|---|---|---|---|---|---|
| Community
|
Methanotroph
|
||||
| CH4 | O2 | 20°C | 30°C | 20°C | 30°C |
| 4.5 | 19.0 | 0.35 ± 0.03 (1) | 0.42 ± 0.03 (1) | 0.27 ± 0.05 (6) | 0.32 ± 0.06 (3) |
| 8.7 | 18.5 | 0.38 ± 0.02 (1) | 0.40 ± 0.04 (0.5) | 0.27 ± 0.06 (5) | 0.30 ± 0.07 (4) |
| 19.4 | 15.5 | 0.36 ± 0.04 (1) | 0.44 ± 0.02 (0.5) | 0.30 ± 0.05 (3) | 0.34 ± 0.07 (2) |
| 20.2b | 14.8 | 0.45 ± 0.03 (0.5) | 0.55 ± 0.03 (0.5) | 0.38 ± 0.06 (3) | 0.45 ± 0.06 (1) |
Average specific growth rate (mean ± standard deviation of three replicates) during the log phase. Data in parentheses indicate lag period in days.
After the first injection, CH4 (20 ml) and O2 (10 ml) were repeatedly injected to maintain low O2 (3 to 5%) and excess CH4 (10 to 15%) concentrations.