TABLE 3.
General nutritional and physiological characteristics of heterotrophic community populationsa
| Characteristic | Het 1 | Het 2 | Het 3 | Het 4 | Het 5 |
|---|---|---|---|---|---|
| Growth on: | |||||
| Rich medium (NA)b | + | + | + | − | + |
| Oligotrophic mediac | + | + | + | + | + |
| Mineral medium (NMS) | − | − | + | − | − |
| Optimal temp (°C) | 30–32 | 28–30 | 28–30 | 20–25 | 28–30 |
| Growth factor requirements | |||||
| Vitaminsd | − | − | + | + | + |
| Amino acidse | + | + | − | + | + |
| Utilization of carbon sources | |||||
| Methanol | + | − | − | + | + |
| Formate | + | + | + | + | + |
| Acetate | + | + | + | − | − |
| Utilization of nitrogen sources | |||||
| NO3− | + | − | + | + | − |
| NH4+ | + | + | + | + | + |
| Urea | + | + | + | + | + |
| LAS transformation | − | − | − | − | − |
| C10SPCf transformation | + | + | + | − | − |
a
Three strains were tentatively identified as members of the genera Blastobacter (Het 1), Pseudomonas (Het 2), and Xanthobacter (Het 3), and two unidentified strains (Het 4 and Het 5) showed similarities to prosthecate bacteria (24).
b
NA, nutrient agar (Difco).
c
PY agar and PYG agar.
d
Biotin, folic acid, and thiamine hydrochloride.
e
Vitamin-free Casamino Acids (Difco).
f
C10SPC, sulfophenyldecanoic acid (a LAS intermediate).