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. 2022 Jun 24;17(6):e0264651. doi: 10.1371/journal.pone.0264651

Fig 7. Representative images from SCLC patient CTCs stained for CCT2.

Fig 7

(A) Representative images of CTCs, based on standard CTC criteria that were CCT2 positive at varying concentrations of the anti-CCT2-PE antibody. (B) Representative images of CTCs from each SCLC patient. Each image was taken from collections of relevant events that were analyzed using standard CTC criteria for the CSS CXC kits as described above. (C) Representative images from CTCs collected using the CTC analysis algorithm (instead of CXC analysis algorithm) where the DAPI signal overlaps with CCT2-PE instead of CK-FLU as in (A, B). Note that images that contain faint CD45 expression are a result of bleed-over from the signal in the PE channel.