Skip to main content
. 2022 Apr 15;73(12):4065–4078. doi: 10.1093/jxb/erac150

Fig. 3.

Fig. 3.

GPAW-induced gene expression associated with dormancy release and germination. (A) Dormancy release of freshly harvested (FH) Arabidopsis thaliana C24 seeds by Air-GPAW (45 min discharge time) and 5 mM KNO3 mimicking the Air-GPAW’s NO3– concentration (see Fig. 2A) as compared with the control (dH2O). (B) Dormancy release of FH C24 seeds by He/O2-GPAW (45 min discharge time) and 300 µM H2O2 mimicking the He/O2-GPAW’s H2O2 concentration (see Fig. 2B). (C) RT–qPCR analyses of seed transcript abundances at 6 h for key genes encoding enzymes for ABA degradation (CYP707A2), GA biosynthesis (GA3OX1), ABA biosynthesis (NECD2/9), and at 24 h for CWRP genes known to be involved in endosperm weakening and germination. Relative mean ±SEM values for the Air-GPAW and NO3– treatments are compared with the 6 h and 24 h samples of the control series (set to 1 for each gene). (D) RT–qPCR analyses of seed transcript abundances for the He/O2-GPAW and H2O2 treatments. Relative mean ±SEM values compared with the 6 h and 24 h controls are presented.