Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene (Drosophila melanogaster)	PRPS	Genbank	P0A717
Strain, strain background (Escherichia coli)	Transetta (DE3)	TransGen Biotech
Recombinant DNA reagent	pRSFDuet-6His	In house
Commercial assay or kit	BCA Protein Concentration Determination Kit (Enhanced)	Beyotime	P0010
Chemical compound, drug	Benzamidine hydrochloride	Sigma-Aldrich	434760–5 G
Chemical compound, drug	Pepstatin A	Sigma-Aldrich	P5318-25MG
Chemical compound, drug	Leupeptin hydrochloride microbial	Sigma/Aldrich	L9783-100MG
Chemical compound, drug	PMSF	MDBio	P006-5g
Chemical compound, drug	Ni-NTA Agarose	QIAGEN	30,250
Chemical compound, drug	Orotic acid	Adamas	01102798 (74736A)
Chemical compound, drug	ATP	Takara	4,041
Chemical compound, drug	D-Ribose 5 phosphate disodium salt	BIOSYNTH CARBOSYNTH	R-5600
Chemical compound, drug	5-phospho-D-ribose 1-diphosphate penta-sodium salt	Sigma	P8296-25 mg
Chemical compound, drug	Adenosine 5'-monophosphate	solarbio	A9860-1
Chemical compound, drug	Adenosine 5'-diphosphate sodium salt	Sigma	A2754-100MG
Other	Nitinol mesh	Zhenjiang Lehua Electronic Technology	M024-Au300-R12/13	Cryo-EM grid preparation
Other	Holey Carbon Film	Quantifoil	R1.2/1.3, 300 Mesh, Cu	Cryo-EM grid preparation
Other	400 mesh reinforced carbon support film	EMCN	BZ31024a	Negative staining
Software, algorithm	UCSF Chimera	https://doi.org/10.1002/jcc.20084		https://www.cgl.ucsf.edu/chimera
Software, algorithm	UCSF Chimera X	https://doi.org/10.1002/pro.3235		https://www.cgl.ucsf.edu/chimerax/
Software, algorithm	Relion	https://doi.org/10.7554/eLife.42166		https://relion.readthedocs.io/en/latest/index.html#
Software, algorithm	Coot	https://doi.org/10.1107/S0907444910007493		https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Software, algorithm	Phenix	https://doi.org/10.1107/S2059798318006551		https://phenix-online.org/