Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 24;11:e77490. doi: 10.7554/eLife.77490

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022, Lyras et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 5. — (a) Representative flow cytometry analysis of tongue leukocytes isolated from Cx3cr1^Gfp^/+ mice at different embryonic (E) and postnatal (p) days of development and 8 week-old adults. Cells were pre-gated for CD45⁺ Epcam^- Gr1^-. (b) Proportions of Cx3cr1-GFP⁺ FOLR2^- (G1) Cx3cr1-GFP⁺ FOLR2⁺ (G2) and Cx3cr1-GFP^- FOLR2⁺ (G3) cells out of CD64⁺ cells. Five to 7 animals per time point were used. (c) 3D reconstructions of whole tongue sagittal sections (left) and magnifications (right) stained with anti-GFP (green), anti-CD31 (blood vessels; red) and anti-TUJ1 (neurons; white) from E14, p0, p3, and p11 Cx3cr1^Gfp^/+ reporter mouse tongues. White dashed line in magnifications (right) represents the barrier between the epithelium layer and the muscle. The experiment was repeated at least 3 times.