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. 2022 Jun 8;31(11-12):322–333. doi: 10.1089/scd.2022.0004

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© Maria Espinosa Gonzalez et al., 2022; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2. — Expression of IL-4, IL-13, and IL-10 cytokines in BM cells differentiating into M-LECP. BM cells were differentiated with CSF-1 followed by LPS as described under Materials and Methods section. (A) Fold change in mRNA expression of the indicated cytokines was calculated based on cDNA concentration-normalized Ct values for qPCR. (B) Conditioned medium of differentiating BM cells collected on days 3 and 6 was analyzed for secreted IL-4, IL-13, and IL-10 proteins using ELISA. All values represent mean ± SD of duplicate of experiments that were reproduced three times. ELISA, enzyme-linked immunosorbent assay.