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. 2022 Jun 8;31(11-12):322–333. doi: 10.1089/scd.2022.0004

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© Maria Espinosa Gonzalez et al., 2022; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 5. — Blockade of IL-10 inhibits immunosuppressive M2 phenotype as well as pro-vascular and lymphatic endothelial differentiation. Total mRNA was isolated from BM cells differentiated with CSF-1 and LPS in the presence of a control and IL-10R blocking antibody. Relative expression of (A) M1 markers, (B) M2 markers, (C) Th2 pathway cytokines and receptors, (D) LEC markers, (E) Vegf family members, (F) NF-κB pathway regulators, (G) selected transcription factors, and (H) selected miscellaneous markers was determined by qPCR. The mean fold increase or decrease ± SD caused by an anti-IL-10R antibody relative to control IgG were calculated based on normalized Ct values. P values determined by Student's t-test are indicated by * ≤ 0.05, ** ≤ 0.01, and *** ≤ 0.001 for differences between expression levels in the presence of anti-IL-10R and control antibodies. All assays were performed in triplicate and reproduced twice.