TABLE 2.
Effect of ethanol on the internalization rate of the maltose transporter in NTH1 wild-type cells and nth1Δ mutant cellsa
| Ethanol concn (%) | Inhibition by ethanol (%) of the internalization rate in:
|
|
|---|---|---|
| NTH1 cells | nth1Δ cells | |
| 0 | 0 | 0 |
| 2 | 55 | 13 |
| 4 | 70 | 28 |
| 6 | 90 | 37 |
Strains YS18 and YSN1 were harvested during growth in YNB medium containing 2% maltose and the required amino acids, suspended in ammonium-free medium in the absence or presence of ethanol at the indicated concentrations, and incubated at 30°C. The rate of internalization, which in these strains started immediately after suspension in ammonium-free medium, was monitored over the first 2 h by measuring maltose transport activity in samples of the suspension taken every 20 min. Data are mean values of two experiments. Differences between the two values were <10%.