Skip to main content
. 2000 Oct;66(10):4456–4461. doi: 10.1128/aem.66.10.4456-4461.2000

TABLE 2.

Effect of ethanol on the internalization rate of the maltose transporter in NTH1 wild-type cells and nth1Δ mutant cellsa

Ethanol concn (%) Inhibition by ethanol (%) of the internalization rate in:
NTH1 cells nth1Δ cells
0 0 0
2 55 13
4 70 28
6 90 37
a

Strains YS18 and YSN1 were harvested during growth in YNB medium containing 2% maltose and the required amino acids, suspended in ammonium-free medium in the absence or presence of ethanol at the indicated concentrations, and incubated at 30°C. The rate of internalization, which in these strains started immediately after suspension in ammonium-free medium, was monitored over the first 2 h by measuring maltose transport activity in samples of the suspension taken every 20 min. Data are mean values of two experiments. Differences between the two values were <10%.