Fig. 2.

Relative specific enzymatic activity of irreversibly adsorbed (A) BtMan2A and (B) CmMan5A. LBG (5.0 mg/ml) was incubated at 37 °C in 50 mM sodium citrate buffer (pH 5) with each enzyme. Relative activities of each irreversibly adsorbed enzyme were measured using pNPM (2 mM in 50 mM sodium citrate buffer, pH5) as a substrate after 1-hour incubation with LBG. The specific activity of the original enzyme solution kept at 37 °C for 1 h without mixing with LBG was taken as 100%. ANOVA analysis for significant activity difference between the same amount of original free and bound enzyme, key: * (p value < 0.05). Values are represented as mean values ± SD (n = 3).