Fig. 8.

Anti-migratory activity of berberine-LCNs in A549 cells. a Wound was created by scratching with a sterile tip on a confluent A549 in six-well plate and treated with or without various doses of berberine-LCNs for 24 h. Photographs were taken on 10 × magnification. b The distance between two edges of wound was measured for 0 and 24 h to calculate the percentage wound closure. c A549 cells were seeded in a transwell chamber and treated with or without various doses of berberine-LCNs. Cells were allow to migrate through the membrane for 24 h. Migrated cells were stained with haematoxylin and eosin and photographed under a microscope. d The cells on the outer layer of the membrane after migration were counted in 5 random positions under a high-power field. Values are expressed as mean ± SEM (n = 3 independent experiments); *p < 0.05, **p < 0.01 vs control (without berberine-LCNs treatment); magnification 20 × . Analysis was performed by one-way ANOVA followed by Dunnett’s multiple comparison test