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. 2022 Jun 24;13:3606. doi: 10.1038/s41467-022-31340-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Representative 5 µm Hematoxylin and Eosin (H&E) microtome sections from gliomas showing that fascicles of spindle-like glioma cells (oncostreams, outlined by the dotted line) are present in a Genetically Engineer Mouse Models of gliomas NPA (NRAS/shAtrx/shP53) and the GL26 intracranial implantable model of glioma. Sections from more than 10 tumors per group were analyzed. Scale bars: 50 μm. B Representative H&E microtome sections of human glioma and human xenografts showing the presence of oncostreams. Sections from more than 5 tumors per group were analyzed. Scale bar: 20 μm. Histograms showing the cellular shape analysis (aspect ratio) (C) and angle orientation (alignment) for the corresponding images (D) show areas of oncostreams (OS) formed by elongated and aligned cells and areas with no oncostreams (No-OS) as rounded and not-aligned cells. E, G Immunostaining shows that tumor cells, mesenchymal cells (ACTA2+), microglia/macrophages (IBA1+ and CD68+), are aligned within, the main orientation axis of oncostreams. Bar graphs show the quantification of ACTA2 + cells, n = 3 (**p = 0.0055) (E), IBA1+ cells, n = 5 (*p = 0.0252) (F) and CD68+ cells, n = 5 (*p = 0.051) (G) within oncostreams areas in NPA tumors. 6–13 areas of oncostreams per tumor section per animal were imaged. Graphs present mean ± SEM; Paired two-sided t-test analysis. Scale bar: 20 μm. H Angle orientation shows the alignment of ACTA2+, IBA1+, and CD68+ cells within oncostreams for the corresponding images. Source data are provided as Source data.