Fig. 5. Cell Surface Glycan Engineering of matriglycan rescues LASV-pseudovirus infection in dystroglycan-deficient cells.

Matriglycan engineered HAP1-DAG1^- cells, and unlabeled DAG1^- and WT controls were incubated for 1 h with rVSV-ΔG-LASV (MOI 1), then washed and incubated for 24 h before quantifying infectivity. a Fluorescence microscopy of eGFP-positive cells 24 h post infection. Three independent experiments were performed with similar results each time. Scale bars represent 500 µm. Also shown are cartoon representations of the glycan structures presented at the surface of indicated cells. b Quantification of GFP-positive cells using a Nexcelom Cellometer. c LASV-pseudovirus infection of cells lacking the classical O-mannosylation pathway (deficient in POMT2), can also be restored by matriglycan labeling with 6 disaccharide repeats. For b, c measurements were taken at n = 3 independent experiments, where bars represent the mean and error bars represent SD. Source data of b, c are provided as a Source Data file.