Fig. 6. Exogenous matriglycan inhibits LASV-pseudovirus infection of wildtype cells in a length and dose dependent manner.

a HAP1-WT cells were infected with rVSV-ΔG-LASV (MOI 1) in the absence and presence of the indicated inhibitor compounds at various concentrations. Infectivity was assessed by fluorescence microscopy by the number of eGFP-positive cells 6 h post infection. The 5-amino-1-pentanol linker compound and the linker compound conjugated to the Xyl-GlcA primer region (0 repeats) were unable to inhibit infection at the highest concentration tested. Measurements were taken at n = 3 independent experiments, where bars represent the mean and error bars represent SD. P-values are indicated as follows: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (One-way ANOVA followed by Dunnett’s Test). b, c Representative fluorescence microscopy images of infected WT cells with b no inhibitor or c 50 µM inhibitor with 6 repeats. Scale bars represent 500 µm. Compound identifiers are defined in Fig. 2 and indicated in parentheses. Source data of a are provided as a Source Data file.