Table 1.

Biolayer interferometry kinetic analysis of AimR receptors binding to aimX operators.

Receptor	DNA operator	KD (M)a	kon (M−1 s−1)	koff (s−1)	X2	R2
AimRSPβ-Ib	aimX-boxSPβ	1.93 × 10−8	3.75 × 106 ± 3.24 × 105	7.26 × 10−2 ± 2.29 × 10−3	3.98	0.99
AimRSPβ-I	aimX-boxKat	1.96 × 10−8	3.98 × 106 ± 4.27 × 105	7.80 × 10−2 ± 2.50 × 10−3	2.79	0.99
AimRSPβ-I	aimX-boxPhi	NBDd
AimRKat	aimX-boxSPβ	2.14 × 10−8	3.43 × 106 ± 3.05 × 105	7.32 × 10−2 ± 2.25 × 10−3	4.78	0.99
AimRKat	aimX-boxKat	2.31 × 10−8	3.26 × 106 ± 2.33 × 105	7.52 × 10−2 ± 1.90 × 10−3	3.35	0.98
AimRKat	aimX-boxPhi	NBD
AimRSPβ−IIc	aimX-boxSPβ	1.16 × 10−7	6.31 × 105 ± 2.88 × 104	7.35 × 10−2 ± 1.23 × 10−3	4.19	0.99
AimRSPβ−II	aimX-boxKat	1.16 × 10−7	6.77 × 105 ± 1.82 × 104	7.71 × 10−2 ± 1.12 × 10−3	1.76	0.99

^aValues are the mean of five measurements.

^bAimR^SPβ-I: AimR^SPβ receptor without tags.

^cAimR^SPβ-II: AimR^SPβ receptor with C-terminal His tag.

^dNBD: not binding detected in the experimental conditions used. K_D > 1 × 10⁻⁶ M.