TABLE 3.

Primers pairs designed for specific amplification of E. lata DNA

Primer pair	Sequence of primer (5′-3′)	Genomic origin	Expected PCR product size (bp)	Annealing temp (°C)
SCA 10A	TAGTGGTGTCAGTGAAAGG	RAPD fragment A10-410a	350	60
SCA 10B	GTGCTAAAGCTTAAAATCCC	RAPD fragment A10-410	350	60
SCB 02A	AATCGATGTGAGAGATGG	RAPD fragment B02-830a
SCB 02B	AGGTCAATGATAGCCAAC	RAPD fragment B02-830	700	60
SCD 18A	GAGTACGTTGGTACAATGG	RAPD fragment D18-530a
SCD 18B	ACTCTCTCTCGTCTTTTGC	RAPD fragment D18-530	450	60
Lata 1	GAGCTACCCTGTAGCCCGCTG	rDNA ITS1 regionb
Lata 2-1	CTATCCGGAGATAGGCTCCC	rDNA ITS2 regionb	302	65
Lata 1	GAGCTACCCTGTAGCCCGCTG	rDNA ITS1 region
Lata 2-2	GACGTCAGCCGTGACACACC	rDNA ITS2 region	385	65
Lata 3	GCCTACCCGCCGGTGGACAC	rDNA ITS1 region
Lata 2-1	CTATCCGGAGATAGGCTCCC	rDNA ITS2 region	281	65

^aAs reported in reference 28. 

^bGenBank accession number AF099911.