TABLE 5.
E. lata diagnosis of 18 diseased grapevines: comparison of results of isolation, PCR identification from crude mycelium after isolation, and direct PCR detection from wood material
| Grapevine | Visual assessment of E lata myceliuma | PCR identification from culturesb | Direct PCR detection from wood shavings with the primer pairc:
|
|||
|---|---|---|---|---|---|---|
| Lata 1- Lata 2.2
|
SCA 10A- SCA 10B
|
|||||
| 1/10 dilution | 1/100 dilution | 1/10 dilution | 1/100 dilution | |||
| 1 | 14 | 1 | 3 | 3 | 3 | 3 |
| 2 | 12 | 1 | 3 | 3 | 3 | 3 |
| 3 | 16 | 1 | 3 | 3 | 3 | 3 |
| 4 | 15 | 1 | 3 | 3 | 3 | 3 |
| 5 | 15 | 1 | 3 | 3 | 3 | 3 |
| 6 | 18 | 1 | 3 | 3 | 3 | 3 |
| 7 | 15 | 1 | 3 | 3 | 3 | 3 |
| 8 | 15 | 1 | 3 | 3 | 3 | 2 |
| 9 | 16 | 1 | 3 | 3 | 3 | 2 |
| 10 | 19 | 1 | 3 | 3 | 3 | 2 |
| 11 | 18 | 1 | 3 | 3 | 3 | 2 |
| 12 | 14 | 1 | 3 | 3 | 2 | 2 |
| 13 | 9 | 1 | 3 | 3 | 2 | 1 |
| 14 | 7 | 1 | 3 | 3 | 2 | 1 |
| 15 | 6 | 1 | 3 | 1 | 1 | 1 |
| 16 | 5 | 1 | 3 | 1 | 1 | 1 |
| 17 | 3 | 1 | 3 | 1 | 1 | 0 |
| 18 | 0 | 1 | 2 | 0 | 1 | 0 |
a
Number of wood chips from which the development of E. lata was observed per 20 wood chips deposited onto culture medium.
b
Number of mycelial samples where E. lata was identified per one sample collected from the culture medium per lesion.
c
Number of wood shavings where E. lata was detected per three wood shavings analyzed.