TABLE 1.
Screening results for Comamonas sp. strain DA001
| Stain | Color | Efficiency | Undesirable effects on:
|
Well retained | |
|---|---|---|---|---|---|
| Cell culturabilitya | Adhesionb | ||||
| Calcein blue AM | Blue | Low | NDc | ND | ND |
| CMAC | Blue | Moderate | No | No | ND |
| DPH | Blue | Low | ND | ND | ND |
| Fluorescamine | Blue-green | High | Yes | Yes | ND |
| Calcein AM | Green | Low | ND | ND | ND |
| CFDA/SE | Green-yellow | High | No | No | Yes |
| CMFDA | Green | High | No | No | No |
| DiA | Green | High | No | No | No |
| Oregon Green 488 | Green | Low | ND | ND | ND |
| SFDA | Green-yellow | Low | No | ND | ND |
| SP-DiOC18(3) | Green | Low | ND | ND | ND |
| SNARF-1 | Red | Low | ND | ND | ND |
| CTC | Red | Low | ND | ND | ND |
| DiI | Red/orange | High | No | No | ND |
| DiIC18(3)-DS | Red/orange | Moderate | ND | ND | ND |
| 6,6-Ph2-DiIC18(3) | Red/orange | High | No | ND | ND |
| Rhodamine B, hexyl ester | Red/orange | Highd | No | ND | ND |
| SP-DiIC18(3) | Red/orange | Moderated | No | No | ND |
| TAMRA/SE | Red | High | No | No | Yes |
a
A decrease in culturability compared to the culturability of unstained cells was considered an undesirable effect.
b
An increase in adhesion of stained cells compared to adhesion of unstained cells was considered undesirable.
c
ND, not determined.
d
Fluorescence faded quickly.