Table 1:
GENOTYPE | N (SEX) | EXPERIMENT | GOAL |
---|---|---|---|
PV;eGFP | 4 (2M, 2F) | 1) PV + eGFP IHC 2) GR + eGFP IHC |
3) Sensitivity and specificity of PV;eGFP line 4) GR expression in PV neurons |
SST;eGFP | 3 (2M, 1F) | 3) SST + eGFP IHC 4) GR + eGFP IHC |
5) Sensitivity and specificity of SST;eGFP line 6) GR expression in SST neurons |
GRfl/fl, wt/wt | 2 (1M, 1M) | VIRUS + GR IHC | Positive control knockout tissue for GR antibody selectivity |
Abbreviations: PV: parvalbumin, SST: somatostatin, eGFP: enhanced green fluorescent protein, GR: glucocorticoid receptor; IHC: immunohistochemistry.