Table 2.
Immunohistochemical Staining Conditions
| Primary Antibody | Immunogen | Source and Catalog No. | RRID | Species | Antibody Concentration and Amplification | Labeled Cell Types | Specificity Determination |
|---|---|---|---|---|---|---|---|
| Beta IV tubulin | Synthetic peptide | Abcam (ab179509) |
AB_2716759 | Rabbit | 1:1000 2° |
RE sus cells |
1 (Child et al., 2018) |
| KI67 | Synthetic peptide from C-terminus of human Ki-67 | Epitomics (4203-1) |
AB_765010 | Rabbit | 1:300 3° |
Proliferating cells |
1 (Holbrook et al., 2011) |
| OMP | Amino acids 1-163 representing full length OMP of human origin | Santa Cruz Biotechnology (sc-365818) |
AB_10842164 | Mouse | 1:100 2° |
Mature OSNs |
1 (Child et al., 2018) |
| OMP | Recombinant full-length protein | Abcam (ab183947) |
AB_2858281 | Rabbit | 1:700 2° |
Mature OSNs | 2 |
| PGP9.5 | Human PGP9.5 protein purified from pathogen free human brain | Cedarlane (RA95101) |
AB_2313685 | Rabbit | 1:7,000 2° |
OSNs |
1 (Holbrook et al., 2011) |
| SOX2 | Transcription factor Sox2 | Invitrogen (14-9811-82) |
AB_11219471 | Rat | 1:300 2° |
Upstream GBCs, HBCs, sus cells |
1 (Child et al., 2018) |
| SOX9 | KLH-conjugated to the C-terminal sequence of human Sox9 | Emd Millipore (AB5535) |
AB_2239761 | Rabbit | 1:100 2° |
Duct cells, gland cells |
1 (Holbrook et al., 2011) |
| TBX21 | Synthetic peptide within Human T-bet/ Tbx21 aa 1-100 (N terminal) | Abcam (ab150440) |
AB_2889209 | Rabbit | 1:500 3° (adult) TSA (fetal) |
Mitral cells |
1 (Child et al., 2018) |
| TBX21 | Full length recombinant mouse T-bet | Abcam (ab91109) |
AB_2050371 | Mouse | 1:100 3° (adult) TSA (fetal) |
Mitral cells | 2 |
| TUJ1 | Rat brain derived microtubules | BioLegend (MMS-435P-250) | AB_2313773 | Mouse | 1:1000 1:2000 (WM) 2° |
OSNs |
1 (Holbrook et al., 2011) |
| TH | SDS-denatured rat tyrosine hydroxylase | Invitrogen (P21962) |
AB_2539844 | Rabbit | 1:1000 3° (adult) TSA (fetal) |
Dopaminergic periglomerular cells | 2 |
| TH | Shared regions of the Tyroxine Hydroxylase gene product between human and mouse | Abcam (ab76442) |
AB_1524535 | Chicken | 1:300 3° (adult) TSA (fetal) |
Dopaminergic periglomerular cells | 2 |
| VGLUT2 | C-terminal region of Mouse VGLUT2 conjugated to KLH | Abcam (ab84103) |
AB_10674784 | Rabbit | 1:500 3° |
Synaptic terminals of OSNs in OB glomeruli | 2 |
Abbreviations: GBCs – global basal cells; HBCs – horizontal basal cells; OB – olfactory bulb; OMP – olfactory marker protein; OSNs – olfactory sensory neurons; RE – respiratory epithelium; TH – tyrosin hydroxylase; TSA – tyramide signal amplification; TUJ1 – ß-tubulin III; VGLUT2 – vesicular glutamate transporter type 2; WM – whole mount; 1 - prior published report; 2 - specificity based on known cell type expression of protein and absence of staining with removal of antibody