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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Kidney Int. 2022 Mar 24;102(1):108–120. doi: 10.1016/j.kint.2022.02.030

Figure 6 |. Growth factor–associated signaling pathways are activated in Six2-Qpc−/− kidneys.

Figure 6 |

(a) Phospho (p)-extracellular signal–regulated kinase (ERK)1/2 and total ERK1/2 protein levels in whole kidney lysates from Cre− littermate control littermate and Six2-Qpc−/− mutants at postnatal day (P) 0. Lower panel: ratio of p-ERK1/2 to ERK1/2 protein levels. (b) Immunoblot analysis of eukaryotic translation initiation factor 4E-binding protein 1 (EIF4EBP1), phospho (p)-EIF4EBP1, ribosomal protein S6 (S6RP), phospho (p)-S6RP, and α-tubulin in whole kidney lysates from Cre− littermate control and Six2-Qpc−/− mutant mice at age P0. Ponceau S staining to assess protein loading. Lower panels: EIF4EBP1/α-tubulin, p-EIF4EBP1/EIF4EBP1, and p-S6RP/S6RP ratios. Relative transcript levels of Eif4ebp1 in Six2-eGFP/cre–expressing progenitor cells isolated from Six2-Qpc+/− control and Six2-Qpc−/− mice at age embryonic day (E) 18.5. (c) Relative expression levels of genes involved in ribosome biogenesis at E18.5; heat map generated by RNA-seq analysis of Six2-eGFP/cre–expressing progenitor cells isolated from heterozygous Six2-Qpc+/− control and Six2-Qpc−/− mutant mice (n = 4 and 5, respectively). Data are expressed as mean ± SEM; Student’s t test, 2-tailed, *P < 0.05, **P < 0.01, ***P < 0.001. 18S, 18S ribosomal RNA.