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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Kidney Int. 2022 Mar 24;102(1):108–120. doi: 10.1016/j.kint.2022.02.030

Figure 7 |. Six2-Qpc−/− kidneys are characterized by amino acid deficiency.

Figure 7 |

(a) Relative expression levels of genes involved in amino acid metabolism analyzed by RNA-seq at age embryonic day 18.5. Six2-eGFP/cre–expressing progenitor cells were isolated from heterozygous Six2-Qpc+/− control and Six2-Qpc−/− mice (n = 4 and 5, respectively). Lower-right panels: expression analysis of genes involved in asparagine synthesis, transport, and metabolism by quantitative real-time polymerase chain reaction; asparagine synthetase (Asns), solute carrier family 7 member 7 (Slc7a7, also known as y+L amino acid transporter 1), and nuclear encoded asparaginyl-tRNA synthetase (Nars). (b) Relative amino acid levels analyzed by mass spectrometry at age postnatal day 0; Six2-Qpc−/− kidneys were compared with Cre− littermate control kidneys (n = 5 and 7, respectively). (c) Relative levels of tricarboxylic acid cycle metabolites by mass spectrometry and metabolically associated nonessential aminoacids. Significantly decreased amino acids (n = 5) in Six2-Qpc−/− kidneys are shown in red. Data are expressed as mean ± SEM; Student’s t test; 2-tailed,*P < 0.05, **P < 0.01, ***P < 0.001. 18S, 18S ribosomal RNA; α-KG, α-ketoglutarate; Rel, relative.