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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Adv Healthc Mater. 2022 Mar 4;11(12):e2102593. doi: 10.1002/adhm.202102593

Fig. 6. In vivo evaluation of the immunomodulatory platform in a rat model of periodontitis.

Fig. 6.

A. Schematics of administration of microparticles loaded with antibiotics and GMSC sEVs connected to the particles via an MMP-2-sensitive linker. B. Comparison of the effect of sEV-microparticles on the suppression of bacterial growth evaluated 8 weeks after microparticle administration. C. Relative mRNA expression levels of pro-inflammatory (IL-1β, IFN-γ, TNF-α) and anti-inflammatory (IL-10) cytokines in the periodontal tissue. FOXP3 mRNA level increased the most significantly in the group treated with the full microparticles containing both GMSC sEVs and minocycline. D. Evaluation of cytokine concentration in the periodontal tissue. Healthy – healthy rats; Untreated – untreated periodontitis; Blank – PLGA microparticles; Minocycline-MP – minocycline-loaded microparticles; Soluble –minocycline and GMSC sEVs administered in solution; Full – PLGA microparticles with GMSC sEVs and loaded with minocycline. All data are presented as mean ± SD (n = 5). E. Concentration of TNF-α and IL-10 in the saliva during 8 weeks after the GMSC sEV-microparticle administration.