Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Common eastern bumblebee, Bombus impatiens	Koppert Biological Systems, Howell, MI, USA	https://www.koppertus.com/
Chemicals, peptides, and recombinant proteins
RNAlater Ice	Invitrogen	AM7030
Methyl salicylate	Sigma Aldrich	M2047
Critical commercial assays
Dynabeads mRNA DIRECT kit	Invitrogen	61011
Deposited data
Bombus impatiens genome	Sadd73	NCBI, BIMP_2.2, GCF_000188095.3
Bombus impatiens brain bulk transcriptomic sequencing	This study	NCBI SRA database PRJNA787650
Bombus impatiens brain segmentation and atlas data	This study	https://github.com/kocherlab/BumblebeeIsolation
Code	This study	https://github.com/kocherlab/BumblebeeIsolation
Software and algorithms
SLEAP	Pereira36	sleap.ai
Nikon Elements	Nikon	https://www.microscope.healthcare.nikon.com/products/software/nis-elements
ITK-SNAP	Yushkevich83	itksnap.org
MotionMapper	Berman35	https://github.com/gordonberman/MotionMapper
TM3’seq data processing	Pallares45	https://github.com/Lufpa/TM3Seq-Pipeline
Trimmomatic	Bolger72	http://www.usadellab.org/cms/?page=trimmomatic
STAR	Dobin74	https://github.com/alexdobin/STAR
SAMtools	Li75	http://www.htslib.org/
DESeq2	Love79	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
Trinotate	Grabherr78	https://github.com/Trinotate/Trinotate.github.io/blob/master/index.asciidoc
topGO	Alexa79	https://bioconductor.org/packages/release/bioc/html/topGO.html
GO-Figure	Reijnders80	https://gitlab.com/evogenlab/GO-Figure
Weighted gene correlation network analysis	Langfelder81	https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/
Correlation by Individual Level Product	Lea82	https://github.com/AmandaJLea/differential_correlation
elastix	Klein84	https://elastix.lumc.nl/
Other
2.8 mm grinding bead	OPS Diagnostics	GBSS 089–5000-11