Figure 1. ERRγ is required for maintaining the pancreatic exocrine gland.

(A) Scheme of experiments. Vehicle or GSK5182 was administered to C57BL/6J mice through an osmotic pump for 1 wk (0.5 mg/day). (B) H&E and immunofluorescence staining of amylase (AMY) in pancreas from vehicle or GSK5182 treated mice. (C) Scheme of experiments. Primary acini isolated from C57BL/6J mice were treated with vehicle or GSK5182 for 48 h. (D) Western blot for amylase and HSP90 in primary acini isolated from C57BL/6J mice. (E) Genomic structure of TAM-induced ERRγ conditional KO (cKO) allele. R26, ROSA26 locus; red triangle, loxP sequence; blue box, exonic regions. (F) PCR analysis of genomic DNA showing the deletion of ERRγ in primary acini from control and ERR cKO mice. (G) Relative gene expression of ERRγ in pancreas from control and ERRγ cKO mice. (H) Gross images of pancreas from control and ERRγ cKO mice 7 d after the final TAM injection. TAM (75 mg/kg) was administered once daily by intraperitoneal injection for 5 consecutive days. Scale bar, 1 cm. (I-J) Body weights (I) and pancreas (J) from control and ERRγ cKO mice. (K) H&E staining (upper panel) and Masson’s trichrome staining (lower panel) of pancreas from control and ERRγ cKO mice. (L) Quantitation of collagen deposited area over total area in trichrome stained pancreas sections. (M) Relative gene expression of fibrosis marker genes in pancreas from control and ERRγ cKO mice. Results were normalized to 36b4. CON, control; cKO, ERRγ cKO. All data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.005 by student’s t-test.