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. Author manuscript; available in PMC: 2022 Dec 8.
Published in final edited form as: Nature. 2022 Jun 8;606(7915):769–775. doi: 10.1038/s41586-022-04816-9

Extended Data Fig. 6: Glycoforms on IgG2 Fc conserved region N-glycans do not contain acetylated sialic acid.

Extended Data Fig. 6:

Lm-specific IgG from ΔActA Lm-primed virgin and pregnant mice was subjected to trypsin or chymotrypsin digestion and then LC-MS/MS analysis. (a) Representative MS/MS fragmentation from the conserved N-linked glycosylation site at position 183 on the Fc region of IgG2b/c (glycopeptide EDYNSTIR). (b) Extracted ion chromatograph of EDYNSTIR with the observed sialic acid (Neu5Gc) glycoforms and the absence of Acetyl-Neu5Gc, which was confirmed for the entire length of the LC-MS run. (c, d) All glycoforms with observed masses for N-glycans on the conserved Fc region of IgG2b/c for virgin (c) and pregnant (d) Lm-specific IgG. Experiment was performed in triplicate with representative (a, b) and combined (c, d) data shown.