Figure 1.
VVLΔTK-STCΔN1L and VVLΔTK-STCΔN1L-mIL-21 replicate in and are cytotoxic to glioma cell lines
(A) GL261 and G422 were infected with VV and cells and supernatant harvested at 24–96 h post-infection (hpi). Virus was titered using TCID50 assay and the mean PFU/cell shown and significance tested using Student’s unpaired t test (n = 3/group). (B) Aliquots of supernatant from (A) were assessed for IL-21 expression using ELISA. Mean values at each time point are shown and significance tested using Student’s unpaired t test (n = 3/group). (C) Virus cytotoxicity to the cell lines was detected by the MTS assay 6 days following infection. The mean EC50 values of the two viruses is shown (n = 3/group) and significance tested using Student’s unpaired t test. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.