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. 2022 Jun 6;26:105–119. doi: 10.1016/j.omto.2022.05.008

Figure 7.

Figure 7

VVLΔTK-STCΔN1L-mIL-21 can be intravenously delivered effectively in GL261 subcutaneous and orthotopic tumor models

Mice with established GL261 tumors were treated with the transient PI3Kδ inhibitor CAL101 3 h before intravenous (i.v.) delivery of virus on days 1, 3, 5, 14, 16, and 18. α-PD1 was delivered intraperitoneally (i.p.) on days 2, 4, and 6. One biological repeat was carried out. (A) Tumor growth was monitored and mean tumor size ± SD is presented. Tumor volume curve for each mouse is shown on the right. (B) The number of tumors eliminated after treatment, without recurrence during the observation period, is shown. (C) Tumor volumes at days 19 and 22 after the first treatment, significance was tested using a one-way ANOVA with Bonferroni post-hoc testing. After day 19, many mice in the PBS group were sacrificed due to the tumor reaching maximum limit as defined in the home office license. On day 22, mice in control treatment groups were lost due to the tumor reaching the maximum limit. (D) Kaplan-Meier Survival analysis of mice in each group. Log rank (Mantel-Cox) tests were used to determine significance. The significance in comparison with CAL101+VVLΔTK-STCΔN1L-mIL-21 + α-PD1 group is noted on the legend (n = 10/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (E) GL261-luc cell line mouse orthotopic models were treated using the same strategy described above (one biological repeat was carried out). Tumor luminescence radiance is shown and mean tumor size ± SD is presented (left). Kaplan-Meier survival analysis of mice in each group. Log rank (Mantel-Cox) tests were used to determine significance (middle). The number of orthotopic tumors eliminated is shown on the right panel. The significance in comparison with CAL101+VVLΔTK-STCΔN1L-mIL-21 + α-PD1 group is noted on the legend (n = 7/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (F) In vivo bioluminescence imaging of each group at five time points post-treatment. Color changes from blue to red with the increase of radiance intensity.