FIG. 1.

Organization of binary vector pBGgHg. pBGgHg is 9.6 kb in size and consists of a pCAMBIA1300 backbone containing the kanamycin resistance (R) gene and the right border (R/B) and left border (L/B) sequences of Agrobacterium T-DNA. The hygromycin resistance and EGFP genes are located between the border sequences, and each is joined to the A. bisporus glyceraldehyde-3-phosphate dehydrogenase promoter (Pgpd) and the cauliflower mosaic virus terminator (35S-3′). Shown are restriction enzyme sites with map distances in kilobases.