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. 2000 Oct;66(10):4510–4513. doi: 10.1128/aem.66.10.4510-4513.2000

FIG. 2.

FIG. 2

Selection of putative hygromycin-resistant transformants of A. bisporus. Pieces of fruiting body gill tissue were cocultivated with (+A) and without (−A) A. tumefaciens strain AGL-1 carrying the vector pBGgHg containing the A. bisporus gpd promoter and hph gene construct. Shown is the appearance of the cultures after 2 weeks on SM with hygromycin at 30 μg/ml.