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Southern blot analysis of DNA isolated from putative hygromycin-resistant transformants of A. bisporus. Genomic DNA (5 to 10 μg) was isolated from broth cultures, digested with SacI, and probed with an ∼1-kb 32P-labeled hph gene sequence. Lanes: 1 to 6, DNA isolated from putative transformants AT1 to AT6, respectively; 7, DNA isolated from nontransformed A. bisporus. The positions of molecular DNA size markers (kilobases) are shown on the right.
