FIG. 4.

PCR analysis of DNA isolated from putative hygromycin-resistant transformants of A. bisporus. PCR amplification was carried out on genomic DNA using primers (gpd-FH and hph-R) defining an ∼970-bp sequence spanning the A. bisporus gpd promoter and the hph gene. Lanes: 1 to 10, DNA isolated from putative transformants AT3, AT4, AT9, AT10, AT11, AT12, AT16, AT19, AT24, and AT31, respectively; 11, negative control with water; 12, DNA isolated from nontransformed A. bisporus; 13, positive control with vector pBGgHg; M, DNA molecular size markers (kilobases).