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Expression of the hygromycin resistance trait in first-generation basidiospores produced from transgenic cultures of A. bisporus. Basidiospores from two transgenic lines (AT3 and AT6) and the nontransformed parental strain (NP) were plated on SM with hygromycin at 50 μg/ml. The viability of the basidiospores of the parental strain was established on SM without antibiotic (data not shown).
