Figure 2.

PHB1 EC-KO mice have no metabolic phenotype on low-fat diet. For all panels, WT and PHB1 EC-KO male littermates (n = 5) were analyzed at 16 weeks of age A: EchoMRI analysis reveals no difference. B: Chow consumption is similar. C: Spontaneous locomotor activity is similar. XTOT, total number of x-axis infrared-beam breaks. D: Body temperature maintenance in mice placed at 4°C is similar. E: VO₂ measured by indirect calorimetry is similar. F: RER (RER = VCO₂/VO₂) is similar. G: Nonfasting glucose concentration in blood. H: GTT: After overnight fasting, mice were injected with glucose (2 g/kg body wt) i.p., and glucose in blood was measured. H: ITT: After 4-h fasting, mice were injected with insulin (0.6 units/kg body wt) i.p., and glucose in blood was measured. In all panels, plotted data are mean ± SEM.